TABLE 5.4 (Continued)

Specific media components, typical concentrations, characteristics,

and functions

Name

Concentration

Function

Characteristics

Albumin

Antioxidant and growth

factor carrier

Rec. or animal component

Fetuin

Cell attachment

Plant or animal component

Fibronectin

Cell attachment

Animal component

Transferrin

7.5 mg/L

Antioxidant, binds iron

Rec. or animal component

Alpha2-macroglobulin

Trypsin inhibitor

Rec. or animal component

Trypsin

Cell detachment, reduces cell

aggregation, supports virus

entry

Rec. or animal component

C

Endothelial cell growth

factor (ECGF)

Mitogen

Rec. or animal component

Epidermal growth

factor (EGF)

Mitogen

Rec. or animal component

Fibroblast growth factor

Mitogen

Rec. or animal component

Insulin-like growth

factors (IGF-1, IGF-2)

Mitogen

Rec. or animal component

Platelet-derived growth

factor (PDGF)

Mitogen & major growth

factor

Rec. or animal component

Interleukin-1 (IL-1)

Induces IL-2 release

Rec. or animal component

Interleukin-6 (IL-6)

Promotes differentiation

Rec. or animal component

Hydrocortisone

Promotes attachment &

proliferation

Steroid

D

Iron, copper, zinc,

selenium

Traces

Enzyme cofactors

Metals, minerals

E

Chlortetracycline

5 mg/L

Gram-pos. and Gram-neg.

bacteria

Inhibitor of protein

synthesis

Gentamicin sulfate

50 mg/L

Gram-pos. and Gram-neg.

bacteria, mycoplasmas

Inhibitor of protein

synthesis

Nystatin

50 mg/L

Fungi and yeast

Inhibitor of cell membrane

function

Penicillin G

100 U/mL

Gram-pos. bacteria

Inhibitor of cell wall

synthesis

Spectinomycin

20 mg/L

Gram-pos. and Gram-neg.

bacteria

Inhibitor of protein

synthesis

F

2-Mercaptoethanol

0−2 mg/L

Reduce disulfide bonds,

antioxidant

Inorganic salts

0.1-6 g/L

Buffer and osmolality

98

Bioprocessing of Viral Vaccines